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KMID : 0644120050180040010
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Journal of Oriental Gynecology
2005 Volume.18 No. 4 p.10 ~ p.23
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Rhizoma Scirpi induced Apoptosis in Human Cervical Carcinoma HeLa Cells
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Kim Ju-Yeoun
Choe Chang-Min
Yoo Sim-Keun
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Abstract
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Purpose : This study is to examine the ability of Rhizoma Scirpi (RS) to induce HeLa cell viability.
Methods : We culture HeLa cell which is human metrocarcinoma cell in D-MEM included 10% fetal bovine serum(Hyclone Laboratories) below 37^{circ}C, 5% CO2. Then we observed apoptosis of log phage cell which is changed cultivation liquid 24 Hours periodically.
Results : 1. RS induces mitochondria membrane potential collapse. 2. P38 MAPK is involved in RS-induced death in HeLa cells. 3. P38 MAPK is involved in RS-induced apoptosis in HeLa cells. 4. P38 MAPK reguates RS-induced caspase-3, -8 and -9 activation in HeLa cells. 5. The inhibition of caspase regulates RS-induced cell death in HeLa cells. 6. RS induces mitochondria membrane potential collapse in HeLa cells. 7. P38 MPK is involved in the regulation of Bcl-2 and Bfu in HeLa cells.8. RS regulates the expression of Bcl-2 and Bax in HeLa cells. 9. SR induces p38 MAPK activation in HeLa cells.
Conclusion : RS induces apoptosis in HeLa cells via p38 MAPK activation.
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KEYWORD
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Rhizoma Scirpi (RS), Bax, mitochondria membrane potential, Bax, mitochondria, apoptosis, HeLa cells
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